Stimulation of the Activity of Cyclic 3', 5'-Nucleotide Phosphodiesterase by Calcium Ion

Cyclic 3':5'-nucleotide phosphodiesterase. Stimulation of bovine brain cytoplasmic enzyme by lysophosphatidylcholine.

Brain cytoplasmic cyclic 3':5'-nucleotide phosphodiesterase (EC 3.1.4.17) requires an endogenous Ca2+-binding protein for ful activity. We now show that lysophosphatidylcholine also effectively enhances activator-deficient phosphodiesterase activity. Stimulation by both ligands was immediate and reversible; both rendered the enzyme more thermally labile, decreased the energy of activation, and ...

3'-cyclic nucleotide 3'-phosphodiesterase

1. A spectrophotometric assay of 2':3'-cyclic nucleotide 3'-phosphodiesterase (EC 3.1.4.37) based on the use of an acid-base indicator and a buffer having identical PKa values is described. The assay is sinmple and rapid; it was particularly convenient for monitoring the enzyme activity at vat :ous stages of purification. 2. Several proteinases were examined for their ability to solubilize 2':3...

Cyclic 3',5'-Nucleotide Phosphodiesterase Activities in Neurospora crassa

Two molecular species of repressible extracellular phosphodiesterases showing cyclic 2',3'and cyclic 3',5'-nucleotide phosphodiesterase activities were detected in mycelial culture media of wild-type Neurospora crassa and purified. The two molecular species were found to be monomeric and polymeric forms of an enzyme constituted of identical subunits having molecular weights of 50,000. This enzy...

Cyclic nucleotide phosphodiesterase 3 signaling complexes.

The superfamily of cyclic nucleotide phosphodiesterases is comprised of 11 gene families. By hydrolyzing cAMP and cGMP, PDEs are major determinants in the regulation of intracellular concentrations of cyclic nucleotides and cyclic nucleotide-dependent signaling pathways. Two PDE3 subfamilies, PDE3A and PDE3B, have been described. PDE3A and PDE3B hydrolyze cAMP and cGMP with high affinity in a m...

A cyclic 3',5'-nucleotide phosphodiesterase from heart with specificity for uridine 3',5'-phosphate.